This kit provides a rapid spin-column method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants.The kit employs two columns: 1) for gDNA purification and 2) for RNA purification utilizing silicon carbide columns (superior for the binding of all RNA sizes including miRNA). The proteins are also purified on the second column after RNA elution. The proteins are eluted in buffer and are ready for downstream application without any further clean up required. The proteins can be quantified directly, used in western blots, ELISA or mass spectrometry.
产品特点
Sequentially purify total RNA (and miRNA), DNA and proteins from a single sample; No sample splitting or need to use phenol or precipitation methods; Purify RNA/DNA/Protein from cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants; Rapid and efficient spin column procedure - all done in 30 minutes; Proteins are purified on column and are soluble in the elution buffer. No further cleaning is required.
保存建议
Protein Loading Dye储存在-20°C。其他溶液密封室温保存,未开封至少保存1年。具体保存情况收到产品后请按说明书存放。